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KMID : 1007520160250000163
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Food Science and Biotechnology
2016 Volume.25 No. 0 p.163 ~ p.168
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Radioprotective Effect of Hesperetin against γ-Irradiationinduced DNA Damage and Immune Dysfunction in Murine Splenocytes
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Kang Jung-Ae
Yoon Seon-Hye
Rho Jong-Kook
Jang Beom-Su
Choi Dae-Seong
Lee Dong-Eun
Byun Eui-Baek
Jeon Jong-Ho
Park Sang-Hyun
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Abstract
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This study was conducted to evaluate the preventive effect of hesperetin against radiationinduced DNA damage and immune dysfunction in murine splenocytes. Isolated splenocytes from BALB/c mice were treated with hesperetin (20, 100, and 500 μM), and then irradiated at a dose of 2 and 4 Gy of γ-irradiation. Exposure to γ-radiation resulted in DNA damage and a reduction of cell viability as well as an elevation of the levels of proinflammatory cytokines, intracellular ROS (reactive oxygen species), and NO (nitric oxide). Hesperetin significantly enhanced the cell viability of the splenocytes compared with the irradiated group. In addition, hesperetin was found to be highly effective in preventing DNA damage as identified by comet and DNA ladder assays. Hesperetin also effectively inhibited proinflammatory cytokines, intracellular ROS, and NO in irradiated splenocytes. In conclusion, hesperetin was shown to be radioprotective against irradiation-induced DNA damage and immune dysfunction in murine splenocytes.
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KEYWORD
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Hesperetin, γ-irradiation, murine splenocytes, DNA damage, immune dysfunction
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